MyBioSource.com's Glutaminase Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: EIA, Immunoassay, ELISA, Immunocytochemistry, Immunofluorescence, and Western Blot. The Glutaminase Antibody was generated using GLS, and Glutaminase as the antigen and it reacts with Human, Mouse, and Rat.
Description
Function: Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain (PubMed:30575854, PubMed:30239721, PubMed:30970188).Lacks catalytic activity.
Post Translational Modifications: Synthesized as a 74-kDa cytosolic precursor which is proteolytically processed by the mitochondrial-processing peptidase (MPP) via a 72-kDa intermediate to yield the mature mitochondrial 68- and 65-kDa subunits.
Subcellular Location: Mitochondrion. Cytoplasm>Cytosol. Note: The 74-kDa cytosolic precursor is translocated into the mitochondria and processed via a 72-kDa intermediate to yield the mature 68- and 65-kDa subunits.Mitochondrion. Mitochondrion matrix. Note: Produced by the proteolytic processing of the 74-kDa cytosolic precursor.Mitochondrion matrix. Note: Produced by the proteolytic processing of the 74-kDa cytosolic precursor.
Tissue Specificity: Isoform 1 and isoform 3 are detected in brain cortex. Isoform 3 is highly expressed in astrocytoma, ganglioglioma and ependymoma. Isoform 1 is highly expressed in brain and kidney, but not detected in liver. Isoform 3 is highly expressed in heart and pancreas, detected at lower levels in placenta, lung, pancreas and kidney, but is not detected in liver. Isoform 2 is expressed in cardiac and skeletal muscle.
Subunit Structure: Homotetramer, dimer of dimers (PubMed:22538822, PubMed:26988803, PubMed:28526749, PubMed:29317493). The tetramers can assemble into rod-like oligomers (in vitro), but the physiological significance of this is not clear (By similarity). Interacts with RAF1 and MAP2K2 (PubMed:22538822). Interacts with ATCAY; the interaction is direct and may control GLS localization, negatively regulating its activity.
Similarity: The C-terminal ANK repeats prevent the assembly of the supra-tetrameric filaments.A highly mobile activation loop at the dimer-dimer interface is important for enzyme activity.Belongs to the glutaminase family